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Single flux quantum (SFQ) logic is a promising technology to replace complementary metal-oxide-semiconductor logic for future exa-scale supercomputing but requires the development of reliable EDA tools that are tailored to the unique characteristics of SFQ circuits, including the need for active splitters to support fanout and clocked logic gates. This article is the first work to present a physical design methodology for inserting hold buffers in SFQ circuits. Our approach is variation-aware, uses common path pessimism removal and incremental placement to minimize the overhead of timing fixes, and can trade off layout area and timing yield. Compared to a previously proposed approach using fixed hold time margins, Monte Carlo simulations show that, averaging across 10 ISCAS’85 benchmark circuits, our proposed method can reduce the number of inserted hold buffers by 8.4% with a 6.2% improvement in timing yield and by 21.9% with a 1.7% improvement in timing yield. 

Small nuclear RNAs (snRNAs) are the basal components of the spliceosome and play crucial roles in splicing. Their biogenesis is spatiotemporally regulated. However, related mechanisms are still poorly understood. Defective in snRNA processing (DSP1) is an essential component of the DSP1 complex that catalyzes plant snRNA 3′-end maturation by cotranscriptional endonucleolytic cleavage of the primary snRNA transcripts (presnRNAs). Here, we show thatDSP1is subjected to alternative splicing in pollens and embryos, resulting in two splicing variants,DSP1α andDSP1β. Unlike DSP1α, DSP1β is not required for presnRNA 3′-end cleavage. Rather, it competes with DSP1α for the interaction with CPSF73-I, the catalytic subunit of the DSP1 complex, which promotes efficient release of CPSF73-I and the DNA-dependent RNA polymerease II (Pol II) from the 3′ end of snRNA loci thereby facilitates snRNA transcription termination, resulting in increased snRNA levels in pollens. Taken together, this study uncovers a mechanism that spatially regulates snRNA accumulation.